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Linked Open Data
1H, 13C and 15N resonance assignments of the Onconase FL-G zymogen
Identificadores del recurso
http://hdl.handle.net/10256/11967
Procedencia
(DUGiDocs. Recerca)

Ficha

Título:
1H, 13C and 15N resonance assignments of the Onconase FL-G zymogen
Tema:
Enginyeria de proteïnes
Protein engineering
Enzims
Enzymes
Descripción:
Onconase® FL-G zymogen is a 120 residue protein produced by circular permutation of the native Onconase® sequence. In this construction, the wild type N- and C-termini are linked by a 16 residue segment and new N- and C-termini are generated at wild type positions R73 and S72. This novel segment linking the native N- and C-termini is designed to obstruct Onconase's® active site and encloses a cleavage site for the HIV-1 protease. As a first step towards the resolution of its 3D structure and the study of its structure-function relationships, we report here the nearly complete NMR 1H, 13C and 15N resonance chemical shift assignments at pH 5.2 and 35 C (BMRB deposit no 17973). The results presented here clearly show that the structure of the wild type Onconase ® is conserved in the FL-G zymogen
This work was supported by the projects, CTQ2008-0080, CTQ2010-21567-C02-02 and BFU2009-06935/ BMC from MICINN and PUG2008A from the Universitat de Girona
Fuente:
© Biomolecular NMR Assignments, 2013, vol. 7, p. 13-15
Articles publicats (D-B)
Idioma:
English
Relación:
info:eu-repo/semantics/altIdentifier/doi/10.1007/s12104-012-9367-0
info:eu-repo/semantics/altIdentifier/issn/1874-2718
info:eu-repo/semantics/altIdentifier/eissn/1874-270X
info:eu-repo/grantAgreement/MICINN//BFU2009-06935/ES/Bases Moleculares Del Plegamiento Y Citotoxicidad De Las Ribonucleasas Pancreaticas. Evaluacion De La Actividad Citotoxica Y Diseño De Estrategias Para Su Control Mediante Splicing Proteico./
Autor/Productor:
Serrano, Soraya
Callís Figueres, Mariona
Vilanova i Brugués, Maria
Benito i Mundet, Antoni
Laurents, Douglas V.
Ribó i Panosa, Marc
Bruix, Marta
Editor:
Springer
Otros colaboradores/productores:
Ministerio de Ciencia e Innovación (Espanya)
Derechos:
Tots els drets reservats
info:eu-repo/semantics/embargoedAccess
Fecha:
info:eu-repo/date/embargoEnd/2026-01-01
2013
Tipo de recurso:
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Formato:
application/pdf

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    2. < dc:creator > Serrano, Soraya </ dc:creator >

    3. < dc:creator > Callís Figueres, Mariona </ dc:creator >

    4. < dc:creator > Vilanova i Brugués, Maria </ dc:creator >

    5. < dc:creator > Benito i Mundet, Antoni </ dc:creator >

    6. < dc:creator > Laurents, Douglas V. </ dc:creator >

    7. < dc:creator > Ribó i Panosa, Marc </ dc:creator >

    8. < dc:creator > Bruix, Marta </ dc:creator >

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    11. < dc:subject > Protein engineering </ dc:subject >

    12. < dc:subject > Enzims </ dc:subject >

    13. < dc:subject > Enzymes </ dc:subject >

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    2. < creator > Serrano, Soraya </ creator >

    3. < creator > Callís Figueres, Mariona </ creator >

    4. < creator > Vilanova i Brugués, Maria </ creator >

    5. < creator > Benito i Mundet, Antoni </ creator >

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    7. < creator > Ribó i Panosa, Marc </ creator >

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