• Català
  • Castellano
  • English
Logo Catalònica
  • Cerca
  • Col·leccions
  • Coneix-nos
  • Ajuda
  • Directori
  • Professionals
Està a:  › Dades de registre
Linked Open Data
'Parallel' and 'antiparallel' tail-clamps increase the efficiency of triplex formation with structured DNA and RNA targets
Identificadors del recurs
http://hdl.handle.net/10256/26011
Procedència
(DUGiDocs. Recerca)

Fitxa

Títol:
'Parallel' and 'antiparallel' tail-clamps increase the efficiency of triplex formation with structured DNA and RNA targets
Descripció:
Sequence-specific triple-helix structures can be formed by parallel and antiparallel DNA clamps interacting with single-stranded DNA or RNA targets. Single-stranded nucleic acid molecules are known to adopt secondary structures that might interfere with intermolecular interactions. We demonstrate the correlation between a secondary structure involving the target a stable stem predicted by in silico folding and experimentally confirmed by thermal stability and competition analyses and an inhibitory effect on triplex formation. We overcame structural impediments by designing a new type of clamp: 'tail-clamps'. A combination of gel-shift, kinetic analysis, UV thermal melting and thermodynamic techniques was used to demonstrate that tail-clamps efficiently form triple helices with a structured target sequence. The performance of parallel and antiparallel tail-clamps was compared: antiparallel tail-clamps had higher binding efficiencies than parallel tail-clamps both with structured DNA and RNA targets. In addition, the reported triplex-stabilizing property of 8- aminopurine residues was confirmed for tail-clamps. Finally, we discuss the possible use of this improved triplex technology as a new tool for applications in molecular biology
Font:
© ChemBioChem, 2005, vol. 6, núm. 6, p. 1034-1042
Articles publicats (D-EQATA)
Nadal i Matamala, Anna Eritja, Ramon Esteve Nuez, Teresa Pla i de Solà-Morales, Maria 2005 'Parallel' and 'antiparallel' tail-clamps increase the efficiency of triplex formation with structured DNA and RNA targets ChemBioChem 6 6 1034 1042
Idioma:
English
Relació:
info:eu-repo/semantics/altIdentifier/doi/10.1002/cbic.200400358
info:eu-repo/semantics/altIdentifier/issn/1439-4227
info:eu-repo/semantics/altIdentifier/eissn/1439-7633
Autor/Productor:
Nadal i Matamala, Anna
Eritja, Ramon
Esteve Nuez, Teresa
Pla i de Solà-Morales, Maria
Editor:
Wiley-VCH Verlag
Drets:
Tots els drets reservats
info:eu-repo/semantics/openAccess
Data:
2005-06-06
Tipus de recurs:
info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
peer reviewed
Format:
9 p.
application/pdf

oai_dc

Descarregar XML

    <?xml version="1.0" encoding="UTF-8" ?>

  1. < oai_dc:dc schemaLocation =" http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd " >

    1. < dc:title > 'Parallel' and 'antiparallel' tail-clamps increase the efficiency of triplex formation with structured DNA and RNA targets </ dc:title >

    2. < dc:creator > Nadal i Matamala, Anna </ dc:creator >

    3. < dc:creator > Eritja, Ramon </ dc:creator >

    4. < dc:creator > Esteve Nuez, Teresa </ dc:creator >

    5. < dc:creator > Pla i de Solà-Morales, Maria </ dc:creator >

    6. < dc:description > Sequence-specific triple-helix structures can be formed by parallel and antiparallel DNA clamps interacting with single-stranded DNA or RNA targets. Single-stranded nucleic acid molecules are known to adopt secondary structures that might interfere with intermolecular interactions. We demonstrate the correlation between a secondary structure involving the target a stable stem predicted by in silico folding and experimentally confirmed by thermal stability and competition analyses and an inhibitory effect on triplex formation. We overcame structural impediments by designing a new type of clamp: 'tail-clamps'. A combination of gel-shift, kinetic analysis, UV thermal melting and thermodynamic techniques was used to demonstrate that tail-clamps efficiently form triple helices with a structured target sequence. The performance of parallel and antiparallel tail-clamps was compared: antiparallel tail-clamps had higher binding efficiencies than parallel tail-clamps both with structured DNA and RNA targets. In addition, the reported triplex-stabilizing property of 8- aminopurine residues was confirmed for tail-clamps. Finally, we discuss the possible use of this improved triplex technology as a new tool for applications in molecular biology </ dc:description >

    7. < dc:date > 2005-06-06 </ dc:date >

    8. < dc:type > info:eu-repo/semantics/article </ dc:type >

    9. < dc:type > info:eu-repo/semantics/acceptedVersion </ dc:type >

    10. < dc:type > peer reviewed </ dc:type >

    11. < dc:identifier > http://hdl.handle.net/10256/26011 </ dc:identifier >

    12. < dc:relation > info:eu-repo/semantics/altIdentifier/doi/10.1002/cbic.200400358 </ dc:relation >

    13. < dc:relation > info:eu-repo/semantics/altIdentifier/issn/1439-4227 </ dc:relation >

    14. < dc:relation > info:eu-repo/semantics/altIdentifier/eissn/1439-7633 </ dc:relation >

    15. < dc:language > eng </ dc:language >

    16. < dc:source > © ChemBioChem, 2005, vol. 6, núm. 6, p. 1034-1042 </ dc:source >

    17. < dc:source > Articles publicats (D-EQATA) </ dc:source >

    18. < dc:rights > Tots els drets reservats </ dc:rights >

    19. < dc:rights > info:eu-repo/semantics/openAccess </ dc:rights >

    20. < dc:format > 9 p. </ dc:format >

    21. < dc:format > application/pdf </ dc:format >

    22. < dc:publisher > Wiley-VCH Verlag </ dc:publisher >

    23. < dc:source > Nadal i Matamala, Anna Eritja, Ramon Esteve Nuez, Teresa Pla i de Solà-Morales, Maria 2005 'Parallel' and 'antiparallel' tail-clamps increase the efficiency of triplex formation with structured DNA and RNA targets ChemBioChem 6 6 1034 1042 </ dc:source >

    </ oai_dc:dc >

didl

Descarregar XML

    <?xml version="1.0" encoding="UTF-8" ?>

  1. < d:DIDL schemaLocation =" urn:mpeg:mpeg21:2002:02-DIDL-NS http://standards.iso.org/ittf/PubliclyAvailableStandards/MPEG-21_schema_files/did/didl.xsd " >

    1. < d:DIDLInfo >

      1. < dcterms:created schemaLocation =" http://purl.org/dc/terms/ http://dublincore.org/schemas/xmls/qdc/dcterms.xsd " > 2025-01-14T10:44:05Z </ dcterms:created >

      </ d:DIDLInfo >

    2. < d:Item id =" hdl_10256_26011 " >

      1. < d:Descriptor >

        1. < d:Statement mimeType =" application/xml; charset=utf-8 " >

          1. < dii:Identifier schemaLocation =" urn:mpeg:mpeg21:2002:01-DII-NS http://standards.iso.org/ittf/PubliclyAvailableStandards/MPEG-21_schema_files/dii/dii.xsd " > urn:hdl:10256/26011 </ dii:Identifier >

          </ d:Statement >

        </ d:Descriptor >

      2. < d:Descriptor >

        1. < d:Statement mimeType =" application/xml; charset=utf-8 " >

          1. < oai_dc:dc schemaLocation =" http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd " >

            1. < dc:title > 'Parallel' and 'antiparallel' tail-clamps increase the efficiency of triplex formation with structured DNA and RNA targets </ dc:title >

            2. < dc:creator > Nadal i Matamala, Anna </ dc:creator >

            3. < dc:creator > Eritja, Ramon </ dc:creator >

            4. < dc:creator > Esteve Nuez, Teresa </ dc:creator >

            5. < dc:creator > Pla i de Solà-Morales, Maria </ dc:creator >

            6. < dc:description > Sequence-specific triple-helix structures can be formed by parallel and antiparallel DNA clamps interacting with single-stranded DNA or RNA targets. Single-stranded nucleic acid molecules are known to adopt secondary structures that might interfere with intermolecular interactions. We demonstrate the correlation between a secondary structure involving the target a stable stem predicted by in silico folding and experimentally confirmed by thermal stability and competition analyses and an inhibitory effect on triplex formation. We overcame structural impediments by designing a new type of clamp: 'tail-clamps'. A combination of gel-shift, kinetic analysis, UV thermal melting and thermodynamic techniques was used to demonstrate that tail-clamps efficiently form triple helices with a structured target sequence. The performance of parallel and antiparallel tail-clamps was compared: antiparallel tail-clamps had higher binding efficiencies than parallel tail-clamps both with structured DNA and RNA targets. In addition, the reported triplex-stabilizing property of 8- aminopurine residues was confirmed for tail-clamps. Finally, we discuss the possible use of this improved triplex technology as a new tool for applications in molecular biology </ dc:description >

            7. < dc:date > 2025-01-14T10:44:05Z </ dc:date >

            8. < dc:date > 2025-01-14T10:44:05Z </ dc:date >

            9. < dc:date > 2005-06-06 </ dc:date >

            10. < dc:type > info:eu-repo/semantics/article </ dc:type >

            11. < dc:identifier > 1439-4227 </ dc:identifier >

            12. < dc:identifier > http://hdl.handle.net/10256/26011 </ dc:identifier >

            13. < dc:identifier > https://doi.org/10.1002/cbic.200400358 </ dc:identifier >

            14. < dc:identifier > 005171 </ dc:identifier >

            15. < dc:identifier > 1439-7633 </ dc:identifier >

            16. < dc:language > eng </ dc:language >

            17. < dc:relation > Versió postprint del document publicat a: https://doi.org/10.1002/cbic.200400358 </ dc:relation >

            18. < dc:relation > © ChemBioChem, 2005, vol. 6, núm. 6, p. 1034-1042 </ dc:relation >

            19. < dc:relation > Articles publicats (D-EQATA) </ dc:relation >

            20. < dc:rights > info:eu-repo/semantics/openAccess </ dc:rights >

            21. < dc:rights > Tots els drets reservats </ dc:rights >

            22. < dc:publisher > Wiley-VCH Verlag </ dc:publisher >

            23. < dc:source > Nadal i Matamala, Anna Eritja, Ramon Esteve Nuez, Teresa Pla i de Solà-Morales, Maria 2005 'Parallel' and 'antiparallel' tail-clamps increase the efficiency of triplex formation with structured DNA and RNA targets ChemBioChem 6 6 1034 1042 </ dc:source >

            </ oai_dc:dc >

          </ d:Statement >

        </ d:Descriptor >

      3. < d:Component id =" 10256_26011_4 " >

        1. < d:Resource mimeType =" application/pdf " ref =" https://dugi-doc.udg.edu/bitstream/10256/26011/4/Nadal-et-al2005ChemBiochem_AM.pdf " />

        </ d:Component >

      </ d:Item >

    </ d:DIDL >

dim

Descarregar XML

    <?xml version="1.0" encoding="UTF-8" ?>

  1. < dim:dim schemaLocation =" http://www.dspace.org/xmlns/dspace/dim http://www.dspace.org/schema/dim.xsd " >

    1. < dim:field element =" contributor " mdschema =" dc " qualifier =" author " > Nadal i Matamala, Anna </ dim:field >

    2. < dim:field element =" contributor " mdschema =" dc " qualifier =" author " > Eritja, Ramon </ dim:field >

    3. < dim:field element =" contributor " mdschema =" dc " qualifier =" author " > Esteve Nuez, Teresa </ dim:field >

    4. < dim:field element =" contributor " mdschema =" dc " qualifier =" author " > Pla i de Solà-Morales, Maria </ dim:field >

    5. < dim:field element =" date " mdschema =" dc " qualifier =" accessioned " > 2025-01-14T10:44:05Z </ dim:field >

    6. < dim:field element =" date " mdschema =" dc " qualifier =" available " > 2025-01-14T10:44:05Z </ dim:field >

    7. < dim:field element =" date " mdschema =" dc " qualifier =" issued " > 2005-06-06 </ dim:field >

    8. < dim:field element =" identifier " mdschema =" dc " qualifier =" issn " > 1439-4227 </ dim:field >

    9. < dim:field element =" identifier " mdschema =" dc " qualifier =" uri " > http://hdl.handle.net/10256/26011 </ dim:field >

    10. < dim:field element =" identifier " mdschema =" dc " qualifier =" doi " > https://doi.org/10.1002/cbic.200400358 </ dim:field >

    11. < dim:field element =" identifier " mdschema =" dc " qualifier =" idgrec " > 005171 </ dim:field >

    12. < dim:field element =" identifier " mdschema =" dc " qualifier =" eissn " > 1439-7633 </ dim:field >

    13. < dim:field element =" description " mdschema =" dc " qualifier =" abstract " > Sequence-specific triple-helix structures can be formed by parallel and antiparallel DNA clamps interacting with single-stranded DNA or RNA targets. Single-stranded nucleic acid molecules are known to adopt secondary structures that might interfere with intermolecular interactions. We demonstrate the correlation between a secondary structure involving the target a stable stem predicted by in silico folding and experimentally confirmed by thermal stability and competition analyses and an inhibitory effect on triplex formation. We overcame structural impediments by designing a new type of clamp: 'tail-clamps'. A combination of gel-shift, kinetic analysis, UV thermal melting and thermodynamic techniques was used to demonstrate that tail-clamps efficiently form triple helices with a structured target sequence. The performance of parallel and antiparallel tail-clamps was compared: antiparallel tail-clamps had higher binding efficiencies than parallel tail-clamps both with structured DNA and RNA targets. In addition, the reported triplex-stabilizing property of 8- aminopurine residues was confirmed for tail-clamps. Finally, we discuss the possible use of this improved triplex technology as a new tool for applications in molecular biology </ dim:field >

    14. < dim:field element =" description " mdschema =" dc " qualifier =" provenance " > Submitted by Usuari Grec Biblioteca (grec-biblio@udg.edu) on 2025-01-14T10:44:05Z No. of bitstreams: 1 005171.pdf: 376883 bytes, checksum: f369cc1845841e34f27d23295f63698f (MD5) </ dim:field >

    15. < dim:field element =" description " mdschema =" dc " qualifier =" provenance " > Made available in DSpace on 2025-01-14T10:44:05Z (GMT). No. of bitstreams: 1 005171.pdf: 376883 bytes, checksum: f369cc1845841e34f27d23295f63698f (MD5) Previous issue date: 2005-06-06 </ dim:field >

    16. < dim:field element =" format " mdschema =" dc " qualifier =" extent " > 9 p. </ dim:field >

    17. < dim:field element =" format " mdschema =" dc " qualifier =" mimetype " > application/pdf </ dim:field >

    18. < dim:field element =" language " mdschema =" dc " qualifier =" iso " > eng </ dim:field >

    19. < dim:field element =" publisher " mdschema =" dc " > Wiley-VCH Verlag </ dim:field >

    20. < dim:field element =" relation " mdschema =" dc " qualifier =" isformatof " > Versió postprint del document publicat a: https://doi.org/10.1002/cbic.200400358 </ dim:field >

    21. < dim:field element =" relation " mdschema =" dc " qualifier =" ispartof " > © ChemBioChem, 2005, vol. 6, núm. 6, p. 1034-1042 </ dim:field >

    22. < dim:field element =" relation " mdschema =" dc " qualifier =" ispartofseries " > Articles publicats (D-EQATA) </ dim:field >

    23. < dim:field element =" rights " mdschema =" dc " > Tots els drets reservats </ dim:field >

    24. < dim:field element =" rights " mdschema =" dc " qualifier =" accessRights " > info:eu-repo/semantics/openAccess </ dim:field >

    25. < dim:field element =" source " mdschema =" dc " > Nadal i Matamala, Anna Eritja, Ramon Esteve Nuez, Teresa Pla i de Solà-Morales, Maria 2005 'Parallel' and 'antiparallel' tail-clamps increase the efficiency of triplex formation with structured DNA and RNA targets ChemBioChem 6 6 1034 1042 </ dim:field >

    26. < dim:field element =" title " mdschema =" dc " > 'Parallel' and 'antiparallel' tail-clamps increase the efficiency of triplex formation with structured DNA and RNA targets </ dim:field >

    27. < dim:field element =" type " mdschema =" dc " > info:eu-repo/semantics/article </ dim:field >

    28. < dim:field element =" type " mdschema =" dc " qualifier =" version " > info:eu-repo/semantics/acceptedVersion </ dim:field >

    29. < dim:field element =" type " mdschema =" dc " qualifier =" peerreviewed " > peer reviewed </ dim:field >

    </ dim:dim >

edm

Descarregar XML

    <?xml version="1.0" encoding="UTF-8" ?>

  1. < rdf:RDF schemaLocation =" http://www.w3.org/1999/02/22-rdf-syntax-ns# http://www.europeana.eu/schemas/edm/EDM.xsd " >

    1. < edm:ProvidedCHO about =" https://catalonica.bnc.cat/catalonicahub/lod/oai:dugi-doc.udg.edu:10256_--_26011#ent0 " >

      1. < dc:creator > Nadal i Matamala, Anna </ dc:creator >

      2. < dc:creator > Eritja, Ramon </ dc:creator >

      3. < dc:creator > Esteve Nuez, Teresa </ dc:creator >

      4. < dc:creator > Pla i de Solà-Morales, Maria </ dc:creator >

      5. < dc:date > 2005-06-06 </ dc:date >

      6. < dc:description > Sequence-specific triple-helix structures can be formed by parallel and antiparallel DNA clamps interacting with single-stranded DNA or RNA targets. Single-stranded nucleic acid molecules are known to adopt secondary structures that might interfere with intermolecular interactions. We demonstrate the correlation between a secondary structure involving the target a stable stem predicted by in silico folding and experimentally confirmed by thermal stability and competition analyses and an inhibitory effect on triplex formation. We overcame structural impediments by designing a new type of clamp: 'tail-clamps'. A combination of gel-shift, kinetic analysis, UV thermal melting and thermodynamic techniques was used to demonstrate that tail-clamps efficiently form triple helices with a structured target sequence. The performance of parallel and antiparallel tail-clamps was compared: antiparallel tail-clamps had higher binding efficiencies than parallel tail-clamps both with structured DNA and RNA targets. In addition, the reported triplex-stabilizing property of 8- aminopurine residues was confirmed for tail-clamps. Finally, we discuss the possible use of this improved triplex technology as a new tool for applications in molecular biology </ dc:description >

      7. < dc:identifier > http://hdl.handle.net/10256/26011 </ dc:identifier >

      8. < dc:language > eng </ dc:language >

      9. < dc:publisher > Wiley-VCH Verlag </ dc:publisher >

      10. < dc:relation > info:eu-repo/semantics/altIdentifier/doi/10.1002/cbic.200400358 </ dc:relation >

      11. < dc:relation > info:eu-repo/semantics/altIdentifier/issn/1439-4227 </ dc:relation >

      12. < dc:relation > info:eu-repo/semantics/altIdentifier/eissn/1439-7633 </ dc:relation >

      13. < dc:rights > Tots els drets reservats </ dc:rights >

      14. < dc:rights > info:eu-repo/semantics/openAccess </ dc:rights >

      15. < dc:source > © ChemBioChem, 2005, vol. 6, núm. 6, p. 1034-1042 </ dc:source >

      16. < dc:source > Articles publicats (D-EQATA) </ dc:source >

      17. < dc:source > Nadal i Matamala, Anna Eritja, Ramon Esteve Nuez, Teresa Pla i de Solà-Morales, Maria 2005 'Parallel' and 'antiparallel' tail-clamps increase the efficiency of triplex formation with structured DNA and RNA targets ChemBioChem 6 6 1034 1042 </ dc:source >

      18. < dc:title > 'Parallel' and 'antiparallel' tail-clamps increase the efficiency of triplex formation with structured DNA and RNA targets </ dc:title >

      19. < dc:type > info:eu-repo/semantics/article </ dc:type >

      20. < dc:type > info:eu-repo/semantics/acceptedVersion </ dc:type >

      21. < dc:type > peer reviewed </ dc:type >

      22. < edm:type > TEXT </ edm:type >

      </ edm:ProvidedCHO >

    2. < ore:Aggregation about =" https://catalonica.bnc.cat/catalonicahub/lod/oai:dugi-doc.udg.edu:10256_--_26011#ent1 " >

      1. < edm:aggregatedCHO resource =" https://catalonica.bnc.cat/catalonicahub/lod/oai:dugi-doc.udg.edu:10256_--_26011#ent0 " />
      2. < edm:dataProvider > DUGiDocs. Recerca </ edm:dataProvider >

      3. < edm:isShownAt resource =" http://hdl.handle.net/10256/26011 " />
      4. < edm:isShownBy resource =" https://dugi-doc.udg.edu/bitstream/10256/26011/4/Nadal-et-al2005ChemBiochem_AM.pdf " />
      5. < edm:provider > Catalònica </ edm:provider >

      6. < edm:rights resource =" http://creativecommons.org/licenses/by-nc-nd/4.0/ " />

      </ ore:Aggregation >

    </ rdf:RDF >

etdms

Descarregar XML

    <?xml version="1.0" encoding="UTF-8" ?>

  1. < thesis schemaLocation =" http://www.ndltd.org/standards/metadata/etdms/1.0/ http://www.ndltd.org/standards/metadata/etdms/1.0/etdms.xsd " >

    1. < title > 'Parallel' and 'antiparallel' tail-clamps increase the efficiency of triplex formation with structured DNA and RNA targets </ title >

    2. < creator > Nadal i Matamala, Anna </ creator >

    3. < creator > Eritja, Ramon </ creator >

    4. < creator > Esteve Nuez, Teresa </ creator >

    5. < creator > Pla i de Solà-Morales, Maria </ creator >

    6. < description > Sequence-specific triple-helix structures can be formed by parallel and antiparallel DNA clamps interacting with single-stranded DNA or RNA targets. Single-stranded nucleic acid molecules are known to adopt secondary structures that might interfere with intermolecular interactions. We demonstrate the correlation between a secondary structure involving the target a stable stem predicted by in silico folding and experimentally confirmed by thermal stability and competition analyses and an inhibitory effect on triplex formation. We overcame structural impediments by designing a new type of clamp: 'tail-clamps'. A combination of gel-shift, kinetic analysis, UV thermal melting and thermodynamic techniques was used to demonstrate that tail-clamps efficiently form triple helices with a structured target sequence. The performance of parallel and antiparallel tail-clamps was compared: antiparallel tail-clamps had higher binding efficiencies than parallel tail-clamps both with structured DNA and RNA targets. In addition, the reported triplex-stabilizing property of 8- aminopurine residues was confirmed for tail-clamps. Finally, we discuss the possible use of this improved triplex technology as a new tool for applications in molecular biology </ description >

    7. < date > 2025-01-14 </ date >

    8. < date > 2025-01-14 </ date >

    9. < date > 2005-06-06 </ date >

    10. < type > info:eu-repo/semantics/article </ type >

    11. < identifier > 1439-4227 </ identifier >

    12. < identifier > http://hdl.handle.net/10256/26011 </ identifier >

    13. < identifier > https://doi.org/10.1002/cbic.200400358 </ identifier >

    14. < identifier > 005171 </ identifier >

    15. < identifier > 1439-7633 </ identifier >

    16. < language > eng </ language >

    17. < relation > Versió postprint del document publicat a: https://doi.org/10.1002/cbic.200400358 </ relation >

    18. < relation > © ChemBioChem, 2005, vol. 6, núm. 6, p. 1034-1042 </ relation >

    19. < relation > Articles publicats (D-EQATA) </ relation >

    20. < rights > info:eu-repo/semantics/openAccess </ rights >

    21. < rights > Tots els drets reservats </ rights >

    22. < publisher > Wiley-VCH Verlag </ publisher >

    23. < source > Nadal i Matamala, Anna Eritja, Ramon Esteve Nuez, Teresa Pla i de Solà-Morales, Maria 2005 'Parallel' and 'antiparallel' tail-clamps increase the efficiency of triplex formation with structured DNA and RNA targets ChemBioChem 6 6 1034 1042 </ source >

    </ thesis >

marc

Descarregar XML

    <?xml version="1.0" encoding="UTF-8" ?>

  1. < record schemaLocation =" http://www.loc.gov/MARC21/slim http://www.loc.gov/standards/marcxml/schema/MARC21slim.xsd " >

    1. < leader > 00925njm 22002777a 4500 </ leader >

    2. < datafield ind1 =" " ind2 =" " tag =" 042 " >

      1. < subfield code =" a " > dc </ subfield >

      </ datafield >

    3. < datafield ind1 =" " ind2 =" " tag =" 720 " >

      1. < subfield code =" a " > Nadal i Matamala, Anna </ subfield >

      2. < subfield code =" e " > author </ subfield >

      </ datafield >

    4. < datafield ind1 =" " ind2 =" " tag =" 720 " >

      1. < subfield code =" a " > Eritja, Ramon </ subfield >

      2. < subfield code =" e " > author </ subfield >

      </ datafield >

    5. < datafield ind1 =" " ind2 =" " tag =" 720 " >

      1. < subfield code =" a " > Esteve Nuez, Teresa </ subfield >

      2. < subfield code =" e " > author </ subfield >

      </ datafield >

    6. < datafield ind1 =" " ind2 =" " tag =" 720 " >

      1. < subfield code =" a " > Pla i de Solà-Morales, Maria </ subfield >

      2. < subfield code =" e " > author </ subfield >

      </ datafield >

    7. < datafield ind1 =" " ind2 =" " tag =" 260 " >

      1. < subfield code =" c " > 2005-06-06 </ subfield >

      </ datafield >

    8. < datafield ind1 =" " ind2 =" " tag =" 520 " >

      1. < subfield code =" a " > Sequence-specific triple-helix structures can be formed by parallel and antiparallel DNA clamps interacting with single-stranded DNA or RNA targets. Single-stranded nucleic acid molecules are known to adopt secondary structures that might interfere with intermolecular interactions. We demonstrate the correlation between a secondary structure involving the target a stable stem predicted by in silico folding and experimentally confirmed by thermal stability and competition analyses and an inhibitory effect on triplex formation. We overcame structural impediments by designing a new type of clamp: 'tail-clamps'. A combination of gel-shift, kinetic analysis, UV thermal melting and thermodynamic techniques was used to demonstrate that tail-clamps efficiently form triple helices with a structured target sequence. The performance of parallel and antiparallel tail-clamps was compared: antiparallel tail-clamps had higher binding efficiencies than parallel tail-clamps both with structured DNA and RNA targets. In addition, the reported triplex-stabilizing property of 8- aminopurine residues was confirmed for tail-clamps. Finally, we discuss the possible use of this improved triplex technology as a new tool for applications in molecular biology </ subfield >

      </ datafield >

    9. < datafield ind1 =" 8 " ind2 =" " tag =" 024 " >

      1. < subfield code =" a " > 1439-4227 </ subfield >

      </ datafield >

    10. < datafield ind1 =" 8 " ind2 =" " tag =" 024 " >

      1. < subfield code =" a " > http://hdl.handle.net/10256/26011 </ subfield >

      </ datafield >

    11. < datafield ind1 =" 8 " ind2 =" " tag =" 024 " >

      1. < subfield code =" a " > https://doi.org/10.1002/cbic.200400358 </ subfield >

      </ datafield >

    12. < datafield ind1 =" 8 " ind2 =" " tag =" 024 " >

      1. < subfield code =" a " > 005171 </ subfield >

      </ datafield >

    13. < datafield ind1 =" 8 " ind2 =" " tag =" 024 " >

      1. < subfield code =" a " > 1439-7633 </ subfield >

      </ datafield >

    14. < datafield ind1 =" 0 " ind2 =" 0 " tag =" 245 " >

      1. < subfield code =" a " > 'Parallel' and 'antiparallel' tail-clamps increase the efficiency of triplex formation with structured DNA and RNA targets </ subfield >

      </ datafield >

    </ record >

mets

Descarregar XML

    <?xml version="1.0" encoding="UTF-8" ?>

  1. < mets ID =" DSpace_ITEM_10256-26011 " OBJID =" hdl:10256/26011 " PROFILE =" DSpace METS SIP Profile 1.0 " TYPE =" DSpace ITEM " schemaLocation =" http://www.loc.gov/METS/ http://www.loc.gov/standards/mets/mets.xsd " >

    1. < metsHdr CREATEDATE =" 2025-02-14T23:22:59Z " >

      1. < agent ROLE =" CUSTODIAN " TYPE =" ORGANIZATION " >

        1. < name > DUGiDocs </ name >

        </ agent >

      </ metsHdr >

    2. < dmdSec ID =" DMD_10256_26011 " >

      1. < mdWrap MDTYPE =" MODS " >

        1. < xmlData schemaLocation =" http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-1.xsd " >

          1. < mods:mods schemaLocation =" http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-1.xsd " >

            1. < mods:name >

              1. < mods:role >

                1. < mods:roleTerm type =" text " > author </ mods:roleTerm >

                </ mods:role >

              2. < mods:namePart > Nadal i Matamala, Anna </ mods:namePart >

              </ mods:name >

            2. < mods:name >

              1. < mods:role >

                1. < mods:roleTerm type =" text " > author </ mods:roleTerm >

                </ mods:role >

              2. < mods:namePart > Eritja, Ramon </ mods:namePart >

              </ mods:name >

            3. < mods:name >

              1. < mods:role >

                1. < mods:roleTerm type =" text " > author </ mods:roleTerm >

                </ mods:role >

              2. < mods:namePart > Esteve Nuez, Teresa </ mods:namePart >

              </ mods:name >

            4. < mods:name >

              1. < mods:role >

                1. < mods:roleTerm type =" text " > author </ mods:roleTerm >

                </ mods:role >

              2. < mods:namePart > Pla i de Solà-Morales, Maria </ mods:namePart >

              </ mods:name >

            5. < mods:extension >

              1. < mods:dateAccessioned encoding =" iso8601 " > 2025-01-14T10:44:05Z </ mods:dateAccessioned >

              </ mods:extension >

            6. < mods:extension >

              1. < mods:dateAvailable encoding =" iso8601 " > 2025-01-14T10:44:05Z </ mods:dateAvailable >

              </ mods:extension >

            7. < mods:originInfo >

              1. < mods:dateIssued encoding =" iso8601 " > 2005-06-06 </ mods:dateIssued >

              </ mods:originInfo >

            8. < mods:identifier type =" issn " > 1439-4227 </ mods:identifier >

            9. < mods:identifier type =" uri " > http://hdl.handle.net/10256/26011 </ mods:identifier >

            10. < mods:identifier type =" doi " > https://doi.org/10.1002/cbic.200400358 </ mods:identifier >

            11. < mods:identifier type =" idgrec " > 005171 </ mods:identifier >

            12. < mods:identifier type =" eissn " > 1439-7633 </ mods:identifier >

            13. < mods:abstract > Sequence-specific triple-helix structures can be formed by parallel and antiparallel DNA clamps interacting with single-stranded DNA or RNA targets. Single-stranded nucleic acid molecules are known to adopt secondary structures that might interfere with intermolecular interactions. We demonstrate the correlation between a secondary structure involving the target a stable stem predicted by in silico folding and experimentally confirmed by thermal stability and competition analyses and an inhibitory effect on triplex formation. We overcame structural impediments by designing a new type of clamp: 'tail-clamps'. A combination of gel-shift, kinetic analysis, UV thermal melting and thermodynamic techniques was used to demonstrate that tail-clamps efficiently form triple helices with a structured target sequence. The performance of parallel and antiparallel tail-clamps was compared: antiparallel tail-clamps had higher binding efficiencies than parallel tail-clamps both with structured DNA and RNA targets. In addition, the reported triplex-stabilizing property of 8- aminopurine residues was confirmed for tail-clamps. Finally, we discuss the possible use of this improved triplex technology as a new tool for applications in molecular biology </ mods:abstract >

            14. < mods:language >

              1. < mods:languageTerm authority =" rfc3066 " > eng </ mods:languageTerm >

              </ mods:language >

            15. < mods:accessCondition type =" useAndReproduction " > Tots els drets reservats </ mods:accessCondition >

            16. < mods:titleInfo >

              1. < mods:title > 'Parallel' and 'antiparallel' tail-clamps increase the efficiency of triplex formation with structured DNA and RNA targets </ mods:title >

              </ mods:titleInfo >

            17. < mods:genre > info:eu-repo/semantics/article </ mods:genre >

            </ mods:mods >

          </ xmlData >

        </ mdWrap >

      </ dmdSec >

    3. < amdSec ID =" TMD_10256_26011 " >

      1. < rightsMD ID =" RIG_10256_26011 " >

        1. < mdWrap MDTYPE =" OTHER " MIMETYPE =" text/plain " OTHERMDTYPE =" DSpaceDepositLicense " >

          1. < binData > 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 </ binData >

          </ mdWrap >

        </ rightsMD >

      </ amdSec >

    4. < amdSec ID =" FO_10256_26011_4 " >

      1. < techMD ID =" TECH_O_10256_26011_4 " >

        1. < mdWrap MDTYPE =" PREMIS " >

          1. < xmlData schemaLocation =" http://www.loc.gov/standards/premis http://www.loc.gov/standards/premis/PREMIS-v1-0.xsd " >

            1. < premis:premis >

              1. < premis:object >

                1. < premis:objectIdentifier >

                  1. < premis:objectIdentifierType > URL </ premis:objectIdentifierType >

                  2. < premis:objectIdentifierValue > https://dugi-doc.udg.edu/bitstream/10256/26011/4/Nadal-et-al2005ChemBiochem_AM.pdf </ premis:objectIdentifierValue >

                  </ premis:objectIdentifier >

                2. < premis:objectCategory > File </ premis:objectCategory >

                3. < premis:objectCharacteristics >

                  1. < premis:fixity >

                    1. < premis:messageDigestAlgorithm > MD5 </ premis:messageDigestAlgorithm >

                    2. < premis:messageDigest > a840d99d5f767546a57ab2e37c7e4374 </ premis:messageDigest >

                    </ premis:fixity >

                  2. < premis:size > 393430 </ premis:size >

                  3. < premis:format >

                    1. < premis:formatDesignation >

                      1. < premis:formatName > application/pdf </ premis:formatName >

                      </ premis:formatDesignation >

                    </ premis:format >

                  </ premis:objectCharacteristics >

                4. < premis:originalName > Nadal-et-al2005ChemBiochem_AM.pdf </ premis:originalName >

                </ premis:object >

              </ premis:premis >

            </ xmlData >

          </ mdWrap >

        </ techMD >

      </ amdSec >

    5. < fileSec >

      1. < fileGrp USE =" ORIGINAL " >

        1. < file ADMID =" FO_10256_26011_4 " CHECKSUM =" a840d99d5f767546a57ab2e37c7e4374 " CHECKSUMTYPE =" MD5 " GROUPID =" GROUP_BITSTREAM_10256_26011_4 " ID =" BITSTREAM_ORIGINAL_10256_26011_4 " MIMETYPE =" application/pdf " SEQ =" 4 " SIZE =" 393430 " >

          1. < FLocat LOCTYPE =" URL " href =" https://dugi-doc.udg.edu/bitstream/10256/26011/4/Nadal-et-al2005ChemBiochem_AM.pdf " type =" simple " />

          </ file >

        </ fileGrp >

      </ fileSec >

    6. < structMap LABEL =" DSpace Object " TYPE =" LOGICAL " >

      1. < div ADMID =" DMD_10256_26011 " TYPE =" DSpace Object Contents " >

        1. < div TYPE =" DSpace BITSTREAM " >

          1. < fptr FILEID =" BITSTREAM_ORIGINAL_10256_26011_4 " />

          </ div >

        </ div >

      </ structMap >

    </ mets >

mods

Descarregar XML

    <?xml version="1.0" encoding="UTF-8" ?>

  1. < mods:mods schemaLocation =" http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-1.xsd " >

    1. < mods:name >

      1. < mods:namePart > Nadal i Matamala, Anna </ mods:namePart >

      </ mods:name >

    2. < mods:name >

      1. < mods:namePart > Eritja, Ramon </ mods:namePart >

      </ mods:name >

    3. < mods:name >

      1. < mods:namePart > Esteve Nuez, Teresa </ mods:namePart >

      </ mods:name >

    4. < mods:name >

      1. < mods:namePart > Pla i de Solà-Morales, Maria </ mods:namePart >

      </ mods:name >

    5. < mods:extension >

      1. < mods:dateAvailable encoding =" iso8601 " > 2025-01-14T10:44:05Z </ mods:dateAvailable >

      </ mods:extension >

    6. < mods:extension >

      1. < mods:dateAccessioned encoding =" iso8601 " > 2025-01-14T10:44:05Z </ mods:dateAccessioned >

      </ mods:extension >

    7. < mods:originInfo >

      1. < mods:dateIssued encoding =" iso8601 " > 2005-06-06 </ mods:dateIssued >

      </ mods:originInfo >

    8. < mods:identifier type =" issn " > 1439-4227 </ mods:identifier >

    9. < mods:identifier type =" uri " > http://hdl.handle.net/10256/26011 </ mods:identifier >

    10. < mods:identifier type =" doi " > https://doi.org/10.1002/cbic.200400358 </ mods:identifier >

    11. < mods:identifier type =" idgrec " > 005171 </ mods:identifier >

    12. < mods:identifier type =" eissn " > 1439-7633 </ mods:identifier >

    13. < mods:abstract > Sequence-specific triple-helix structures can be formed by parallel and antiparallel DNA clamps interacting with single-stranded DNA or RNA targets. Single-stranded nucleic acid molecules are known to adopt secondary structures that might interfere with intermolecular interactions. We demonstrate the correlation between a secondary structure involving the target a stable stem predicted by in silico folding and experimentally confirmed by thermal stability and competition analyses and an inhibitory effect on triplex formation. We overcame structural impediments by designing a new type of clamp: 'tail-clamps'. A combination of gel-shift, kinetic analysis, UV thermal melting and thermodynamic techniques was used to demonstrate that tail-clamps efficiently form triple helices with a structured target sequence. The performance of parallel and antiparallel tail-clamps was compared: antiparallel tail-clamps had higher binding efficiencies than parallel tail-clamps both with structured DNA and RNA targets. In addition, the reported triplex-stabilizing property of 8- aminopurine residues was confirmed for tail-clamps. Finally, we discuss the possible use of this improved triplex technology as a new tool for applications in molecular biology </ mods:abstract >

    14. < mods:language >

      1. < mods:languageTerm > eng </ mods:languageTerm >

      </ mods:language >

    15. < mods:accessCondition type =" useAndReproduction " > info:eu-repo/semantics/openAccess </ mods:accessCondition >

    16. < mods:accessCondition type =" useAndReproduction " > Tots els drets reservats </ mods:accessCondition >

    17. < mods:titleInfo >

      1. < mods:title > 'Parallel' and 'antiparallel' tail-clamps increase the efficiency of triplex formation with structured DNA and RNA targets </ mods:title >

      </ mods:titleInfo >

    18. < mods:genre > info:eu-repo/semantics/article </ mods:genre >

    </ mods:mods >

oai_datacite

Descarregar XML

    <?xml version="1.0" encoding="UTF-8" ?>

  1. < datacite:resource schemaLocation =" http://datacite.org/schema/kernel-4 http://schema.datacite.org/meta/kernel-4-2/metadata.xsd " >

    1. < datacite:identifier identifierType =" Handle " > http://hdl.handle.net/10256/26011 </ datacite:identifier >

    2. < datacite:titles >

      1. < datacite:title > 'Parallel' and 'antiparallel' tail-clamps increase the efficiency of triplex formation with structured DNA and RNA targets </ datacite:title >

      </ datacite:titles >

    3. < datacite:creators >

      1. < datacite:creator >

        1. < datacite:creatorName > Nadal i Matamala, Anna </ datacite:creatorName >

        2. < datacite:nameIdentifier nameIdentifierScheme =" ORCID " schemeURI =" http://orcid.org/ " > 0000-0003-1917-9277 </ datacite:nameIdentifier >

        </ datacite:creator >

      2. < datacite:creator >

        1. < datacite:creatorName > Eritja, Ramon </ datacite:creatorName >

        </ datacite:creator >

      3. < datacite:creator >

        1. < datacite:creatorName > Esteve Nuez, Teresa </ datacite:creatorName >

        </ datacite:creator >

      4. < datacite:creator >

        1. < datacite:creatorName > Pla i de Solà-Morales, Maria </ datacite:creatorName >

        2. < datacite:nameIdentifier nameIdentifierScheme =" ORCID " schemeURI =" http://orcid.org/ " > 0000-0003-4382-5494 </ datacite:nameIdentifier >

        </ datacite:creator >

      </ datacite:creators >

    4. < datacite:contributors />
    5. < datacite:descriptions >

      1. < datacite:description descriptionType =" Abstract " > Sequence-specific triple-helix structures can be formed by parallel and antiparallel DNA clamps interacting with single-stranded DNA or RNA targets. Single-stranded nucleic acid molecules are known to adopt secondary structures that might interfere with intermolecular interactions. We demonstrate the correlation between a secondary structure involving the target a stable stem predicted by in silico folding and experimentally confirmed by thermal stability and competition analyses and an inhibitory effect on triplex formation. We overcame structural impediments by designing a new type of clamp: 'tail-clamps'. A combination of gel-shift, kinetic analysis, UV thermal melting and thermodynamic techniques was used to demonstrate that tail-clamps efficiently form triple helices with a structured target sequence. The performance of parallel and antiparallel tail-clamps was compared: antiparallel tail-clamps had higher binding efficiencies than parallel tail-clamps both with structured DNA and RNA targets. In addition, the reported triplex-stabilizing property of 8- aminopurine residues was confirmed for tail-clamps. Finally, we discuss the possible use of this improved triplex technology as a new tool for applications in molecular biology </ datacite:description >

      </ datacite:descriptions >

    6. < datacite:dates >

      1. < datacite:date dateType =" Issued " > 2005-06-06 </ datacite:date >

      </ datacite:dates >

    7. < datacite:publicationYear > 2005 </ datacite:publicationYear >

    8. < datacite:resourceType resourceTypeGeneral =" Dataset " />
    9. < datacite:languages >

      1. < datacite:language > eng </ datacite:language >

      </ datacite:languages >

    10. < datacite:relatedIdentifiers >

      1. < datacite:relatedIdentifier relatedIdentifierType =" URL " relationType =" IsSupplementTo " > 1439-4227 </ datacite:relatedIdentifier >

      2. < datacite:relatedIdentifier relatedIdentifierType =" DOI " relationType =" IsSupplementTo " > https://doi.org/10.1002/cbic.200400358 </ datacite:relatedIdentifier >

      3. < datacite:relatedIdentifier relatedIdentifierType =" URL " relationType =" IsSupplementTo " > 005171 </ datacite:relatedIdentifier >

      4. < datacite:relatedIdentifier relatedIdentifierType =" URL " relationType =" IsSupplementTo " > 1439-7633 </ datacite:relatedIdentifier >

      </ datacite:relatedIdentifiers >

    11. < datacite:rightsList >

      1. < datacite:rights > Tots els drets reservats </ datacite:rights >

      2. < datacite:rights rightsURI =" info:eu-repo/semantics/openAccess " > info:eu-repo/semantics/openAccess </ datacite:rights >

      </ datacite:rightsList >

    12. < datacite:formats >

      1. < datacite:format > application/pdf </ datacite:format >

      </ datacite:formats >

    13. < datacite:sizes >

      1. < datacite:size />

      </ datacite:sizes >

    14. < datacite:publisher > Wiley-VCH Verlag </ datacite:publisher >

    </ datacite:resource >

ore

Descarregar XML

    <?xml version="1.0" encoding="UTF-8" ?>

  1. < atom:entry schemaLocation =" http://www.w3.org/2005/Atom http://www.kbcafe.com/rss/atom.xsd.xml " >

    1. < atom:id > http://hdl.handle.net/10256/26011/ore.xml </ atom:id >

    2. < atom:link href =" http://hdl.handle.net/10256/26011 " rel =" alternate " />
    3. < atom:link href =" http://hdl.handle.net/10256/26011/ore.xml " rel =" http://www.openarchives.org/ore/terms/describes " />
    4. < atom:link href =" http://hdl.handle.net/10256/26011/ore.xml#atom " rel =" self " type =" application/atom+xml " />
    5. < atom:published > 2025-01-14T10:44:05Z </ atom:published >

    6. < atom:updated > 2025-01-14T10:44:05Z </ atom:updated >

    7. < atom:source >

      1. < atom:generator > DUGiDocs </ atom:generator >

      </ atom:source >

    8. < atom:title > 'Parallel' and 'antiparallel' tail-clamps increase the efficiency of triplex formation with structured DNA and RNA targets </ atom:title >

    9. < atom:author >

      1. < atom:name > Nadal i Matamala, Anna </ atom:name >

      </ atom:author >

    10. < atom:author >

      1. < atom:name > Eritja, Ramon </ atom:name >

      </ atom:author >

    11. < atom:author >

      1. < atom:name > Esteve Nuez, Teresa </ atom:name >

      </ atom:author >

    12. < atom:author >

      1. < atom:name > Pla i de Solà-Morales, Maria </ atom:name >

      </ atom:author >

    13. < atom:category label =" Aggregation " scheme =" http://www.openarchives.org/ore/terms/ " term =" http://www.openarchives.org/ore/terms/Aggregation " />
    14. < atom:category scheme =" http://www.openarchives.org/ore/atom/modified " term =" 2025-01-14T10:44:05Z " />
    15. < atom:category label =" DSpace Item " scheme =" http://www.dspace.org/objectModel/ " term =" DSpaceItem " />
    16. < atom:link href =" https://dugi-doc.udg.edu/bitstream/10256/26011/4/Nadal-et-al2005ChemBiochem_AM.pdf " length =" 393430 " rel =" http://www.openarchives.org/ore/terms/aggregates " title =" Nadal-et-al2005ChemBiochem_AM.pdf " type =" application/pdf " />
    17. < oreatom:triples >

      1. < rdf:Description about =" http://hdl.handle.net/10256/26011/ore.xml#atom " >

        1. < rdf:type resource =" http://www.dspace.org/objectModel/DSpaceItem " />
        2. < dcterms:modified > 2025-01-14T10:44:05Z </ dcterms:modified >

        </ rdf:Description >

      2. < rdf:Description about =" https://dugi-doc.udg.edu/bitstream/10256/26011/4/Nadal-et-al2005ChemBiochem_AM.pdf " >

        1. < rdf:type resource =" http://www.dspace.org/objectModel/DSpaceBitstream " />
        2. < dcterms:description > ORIGINAL </ dcterms:description >

        </ rdf:Description >

      3. < rdf:Description about =" https://dugi-doc.udg.edu/bitstream/10256/26011/3/mets.xml " >

        1. < rdf:type resource =" http://www.dspace.org/objectModel/DSpaceBitstream " />
        2. < dcterms:description > SWORD </ dcterms:description >

        </ rdf:Description >

      4. < rdf:Description about =" https://dugi-doc.udg.edu/bitstream/10256/26011/2/license.txt " >

        1. < rdf:type resource =" http://www.dspace.org/objectModel/DSpaceBitstream " />
        2. < dcterms:description > LICENSE </ dcterms:description >

        </ rdf:Description >

      </ oreatom:triples >

    </ atom:entry >

qdc

Descarregar XML

    <?xml version="1.0" encoding="UTF-8" ?>

  1. < qdc:qualifieddc schemaLocation =" http://purl.org/dc/elements/1.1/ http://dublincore.org/schemas/xmls/qdc/2006/01/06/dc.xsd http://purl.org/dc/terms/ http://dublincore.org/schemas/xmls/qdc/2006/01/06/dcterms.xsd http://dspace.org/qualifieddc/ http://www.ukoln.ac.uk/metadata/dcmi/xmlschema/qualifieddc.xsd " >

    1. < dc:title > 'Parallel' and 'antiparallel' tail-clamps increase the efficiency of triplex formation with structured DNA and RNA targets </ dc:title >

    2. < dc:creator > Nadal i Matamala, Anna </ dc:creator >

    3. < dc:creator > Eritja, Ramon </ dc:creator >

    4. < dc:creator > Esteve Nuez, Teresa </ dc:creator >

    5. < dc:creator > Pla i de Solà-Morales, Maria </ dc:creator >

    6. < dcterms:abstract > Sequence-specific triple-helix structures can be formed by parallel and antiparallel DNA clamps interacting with single-stranded DNA or RNA targets. Single-stranded nucleic acid molecules are known to adopt secondary structures that might interfere with intermolecular interactions. We demonstrate the correlation between a secondary structure involving the target a stable stem predicted by in silico folding and experimentally confirmed by thermal stability and competition analyses and an inhibitory effect on triplex formation. We overcame structural impediments by designing a new type of clamp: 'tail-clamps'. A combination of gel-shift, kinetic analysis, UV thermal melting and thermodynamic techniques was used to demonstrate that tail-clamps efficiently form triple helices with a structured target sequence. The performance of parallel and antiparallel tail-clamps was compared: antiparallel tail-clamps had higher binding efficiencies than parallel tail-clamps both with structured DNA and RNA targets. In addition, the reported triplex-stabilizing property of 8- aminopurine residues was confirmed for tail-clamps. Finally, we discuss the possible use of this improved triplex technology as a new tool for applications in molecular biology </ dcterms:abstract >

    7. < dcterms:issued > 2005-06-06 </ dcterms:issued >

    8. < dc:type > info:eu-repo/semantics/article </ dc:type >

    9. < dc:identifier > 1439-4227 </ dc:identifier >

    10. < dc:identifier > http://hdl.handle.net/10256/26011 </ dc:identifier >

    11. < dc:identifier > https://doi.org/10.1002/cbic.200400358 </ dc:identifier >

    12. < dc:identifier > 005171 </ dc:identifier >

    13. < dc:identifier > 1439-7633 </ dc:identifier >

    14. < dc:language > eng </ dc:language >

    15. < dc:relation > Versió postprint del document publicat a: https://doi.org/10.1002/cbic.200400358 </ dc:relation >

    16. < dc:relation > © ChemBioChem, 2005, vol. 6, núm. 6, p. 1034-1042 </ dc:relation >

    17. < dc:relation > Articles publicats (D-EQATA) </ dc:relation >

    18. < dc:rights > info:eu-repo/semantics/openAccess </ dc:rights >

    19. < dc:rights > Tots els drets reservats </ dc:rights >

    20. < dc:publisher > Wiley-VCH Verlag </ dc:publisher >

    21. < dc:source > Nadal i Matamala, Anna Eritja, Ramon Esteve Nuez, Teresa Pla i de Solà-Morales, Maria 2005 'Parallel' and 'antiparallel' tail-clamps increase the efficiency of triplex formation with structured DNA and RNA targets ChemBioChem 6 6 1034 1042 </ dc:source >

    </ qdc:qualifieddc >

rdf

Descarregar XML

    <?xml version="1.0" encoding="UTF-8" ?>

  1. < rdf:RDF schemaLocation =" http://www.openarchives.org/OAI/2.0/rdf/ http://www.openarchives.org/OAI/2.0/rdf.xsd " >

    1. < ow:Publication about =" oai:dugi-doc.udg.edu:10256/26011 " >

      1. < dc:title > 'Parallel' and 'antiparallel' tail-clamps increase the efficiency of triplex formation with structured DNA and RNA targets </ dc:title >

      2. < dc:creator > Nadal i Matamala, Anna </ dc:creator >

      3. < dc:creator > Eritja, Ramon </ dc:creator >

      4. < dc:creator > Esteve Nuez, Teresa </ dc:creator >

      5. < dc:creator > Pla i de Solà-Morales, Maria </ dc:creator >

      6. < dc:description > Sequence-specific triple-helix structures can be formed by parallel and antiparallel DNA clamps interacting with single-stranded DNA or RNA targets. Single-stranded nucleic acid molecules are known to adopt secondary structures that might interfere with intermolecular interactions. We demonstrate the correlation between a secondary structure involving the target a stable stem predicted by in silico folding and experimentally confirmed by thermal stability and competition analyses and an inhibitory effect on triplex formation. We overcame structural impediments by designing a new type of clamp: 'tail-clamps'. A combination of gel-shift, kinetic analysis, UV thermal melting and thermodynamic techniques was used to demonstrate that tail-clamps efficiently form triple helices with a structured target sequence. The performance of parallel and antiparallel tail-clamps was compared: antiparallel tail-clamps had higher binding efficiencies than parallel tail-clamps both with structured DNA and RNA targets. In addition, the reported triplex-stabilizing property of 8- aminopurine residues was confirmed for tail-clamps. Finally, we discuss the possible use of this improved triplex technology as a new tool for applications in molecular biology </ dc:description >

      7. < dc:date > 2025-01-14T10:44:05Z </ dc:date >

      8. < dc:date > 2025-01-14T10:44:05Z </ dc:date >

      9. < dc:date > 2005-06-06 </ dc:date >

      10. < dc:type > info:eu-repo/semantics/article </ dc:type >

      11. < dc:identifier > 1439-4227 </ dc:identifier >

      12. < dc:identifier > http://hdl.handle.net/10256/26011 </ dc:identifier >

      13. < dc:identifier > https://doi.org/10.1002/cbic.200400358 </ dc:identifier >

      14. < dc:identifier > 005171 </ dc:identifier >

      15. < dc:identifier > 1439-7633 </ dc:identifier >

      16. < dc:language > eng </ dc:language >

      17. < dc:relation > Versió postprint del document publicat a: https://doi.org/10.1002/cbic.200400358 </ dc:relation >

      18. < dc:relation > © ChemBioChem, 2005, vol. 6, núm. 6, p. 1034-1042 </ dc:relation >

      19. < dc:relation > Articles publicats (D-EQATA) </ dc:relation >

      20. < dc:rights > info:eu-repo/semantics/openAccess </ dc:rights >

      21. < dc:rights > Tots els drets reservats </ dc:rights >

      22. < dc:publisher > Wiley-VCH Verlag </ dc:publisher >

      23. < dc:source > Nadal i Matamala, Anna Eritja, Ramon Esteve Nuez, Teresa Pla i de Solà-Morales, Maria 2005 'Parallel' and 'antiparallel' tail-clamps increase the efficiency of triplex formation with structured DNA and RNA targets ChemBioChem 6 6 1034 1042 </ dc:source >

      </ ow:Publication >

    </ rdf:RDF >

xoai

Descarregar XML

    <?xml version="1.0" encoding="UTF-8" ?>

  1. < metadata schemaLocation =" http://www.lyncode.com/xoai http://www.lyncode.com/xsd/xoai.xsd " >

    1. < element name =" dc " >

      1. < element name =" contributor " >

        1. < element name =" author " >

          1. < element name =" none " >

            1. < field name =" value " > Nadal i Matamala, Anna </ field >

            2. < field name =" value " > Eritja, Ramon </ field >

            3. < field name =" value " > Esteve Nuez, Teresa </ field >

            4. < field name =" value " > Pla i de Solà-Morales, Maria </ field >

            </ element >

          </ element >

        </ element >

      2. < element name =" date " >

        1. < element name =" accessioned " >

          1. < element name =" none " >

            1. < field name =" value " > 2025-01-14T10:44:05Z </ field >

            </ element >

          </ element >

        2. < element name =" available " >

          1. < element name =" none " >

            1. < field name =" value " > 2025-01-14T10:44:05Z </ field >

            </ element >

          </ element >

        3. < element name =" issued " >

          1. < element name =" none " >

            1. < field name =" value " > 2005-06-06 </ field >

            </ element >

          </ element >

        </ element >

      3. < element name =" identifier " >

        1. < element name =" issn " >

          1. < element name =" none " >

            1. < field name =" value " > 1439-4227 </ field >

            </ element >

          </ element >

        2. < element name =" uri " >

          1. < element name =" none " >

            1. < field name =" value " > http://hdl.handle.net/10256/26011 </ field >

            </ element >

          </ element >

        3. < element name =" doi " >

          1. < element name =" none " >

            1. < field name =" value " > https://doi.org/10.1002/cbic.200400358 </ field >

            </ element >

          </ element >

        4. < element name =" idgrec " >

          1. < element name =" none " >

            1. < field name =" value " > 005171 </ field >

            </ element >

          </ element >

        5. < element name =" eissn " >

          1. < element name =" none " >

            1. < field name =" value " > 1439-7633 </ field >

            </ element >

          </ element >

        </ element >

      4. < element name =" description " >

        1. < element name =" abstract " >

          1. < element name =" none " >

            1. < field name =" value " > Sequence-specific triple-helix structures can be formed by parallel and antiparallel DNA clamps interacting with single-stranded DNA or RNA targets. Single-stranded nucleic acid molecules are known to adopt secondary structures that might interfere with intermolecular interactions. We demonstrate the correlation between a secondary structure involving the target a stable stem predicted by in silico folding and experimentally confirmed by thermal stability and competition analyses and an inhibitory effect on triplex formation. We overcame structural impediments by designing a new type of clamp: 'tail-clamps'. A combination of gel-shift, kinetic analysis, UV thermal melting and thermodynamic techniques was used to demonstrate that tail-clamps efficiently form triple helices with a structured target sequence. The performance of parallel and antiparallel tail-clamps was compared: antiparallel tail-clamps had higher binding efficiencies than parallel tail-clamps both with structured DNA and RNA targets. In addition, the reported triplex-stabilizing property of 8- aminopurine residues was confirmed for tail-clamps. Finally, we discuss the possible use of this improved triplex technology as a new tool for applications in molecular biology </ field >

            </ element >

          </ element >

        2. < element name =" provenance " >

          1. < element name =" none " >

            1. < field name =" value " > Submitted by Usuari Grec Biblioteca (grec-biblio@udg.edu) on 2025-01-14T10:44:05Z No. of bitstreams: 1 005171.pdf: 376883 bytes, checksum: f369cc1845841e34f27d23295f63698f (MD5) </ field >

            2. < field name =" value " > Made available in DSpace on 2025-01-14T10:44:05Z (GMT). No. of bitstreams: 1 005171.pdf: 376883 bytes, checksum: f369cc1845841e34f27d23295f63698f (MD5) Previous issue date: 2005-06-06 </ field >

            </ element >

          </ element >

        </ element >

      5. < element name =" format " >

        1. < element name =" extent " >

          1. < element name =" none " >

            1. < field name =" value " > 9 p. </ field >

            </ element >

          </ element >

        2. < element name =" mimetype " >

          1. < element name =" none " >

            1. < field name =" value " > application/pdf </ field >

            </ element >

          </ element >

        </ element >

      6. < element name =" language " >

        1. < element name =" iso " >

          1. < element name =" none " >

            1. < field name =" value " > eng </ field >

            </ element >

          </ element >

        </ element >

      7. < element name =" publisher " >

        1. < element name =" none " >

          1. < field name =" value " > Wiley-VCH Verlag </ field >

          </ element >

        </ element >

      8. < element name =" relation " >

        1. < element name =" isformatof " >

          1. < element name =" none " >

            1. < field name =" value " > Versió postprint del document publicat a: https://doi.org/10.1002/cbic.200400358 </ field >

            </ element >

          </ element >

        2. < element name =" ispartof " >

          1. < element name =" none " >

            1. < field name =" value " > © ChemBioChem, 2005, vol. 6, núm. 6, p. 1034-1042 </ field >

            </ element >

          </ element >

        3. < element name =" ispartofseries " >

          1. < element name =" none " >

            1. < field name =" value " > Articles publicats (D-EQATA) </ field >

            </ element >

          </ element >

        </ element >

      9. < element name =" rights " >

        1. < element name =" none " >

          1. < field name =" value " > Tots els drets reservats </ field >

          </ element >

        2. < element name =" accessRights " >

          1. < element name =" none " >

            1. < field name =" value " > info:eu-repo/semantics/openAccess </ field >

            </ element >

          </ element >

        </ element >

      10. < element name =" source " >

        1. < element name =" none " >

          1. < field name =" value " > Nadal i Matamala, Anna Eritja, Ramon Esteve Nuez, Teresa Pla i de Solà-Morales, Maria 2005 'Parallel' and 'antiparallel' tail-clamps increase the efficiency of triplex formation with structured DNA and RNA targets ChemBioChem 6 6 1034 1042 </ field >

          </ element >

        </ element >

      11. < element name =" title " >

        1. < element name =" none " >

          1. < field name =" value " > 'Parallel' and 'antiparallel' tail-clamps increase the efficiency of triplex formation with structured DNA and RNA targets </ field >

          </ element >

        </ element >

      12. < element name =" type " >

        1. < element name =" none " >

          1. < field name =" value " > info:eu-repo/semantics/article </ field >

          </ element >

        2. < element name =" version " >

          1. < element name =" none " >

            1. < field name =" value " > info:eu-repo/semantics/acceptedVersion </ field >

            </ element >

          </ element >

        3. < element name =" peerreviewed " >

          1. < element name =" none " >

            1. < field name =" value " > peer reviewed </ field >

            </ element >

          </ element >

        </ element >

      </ element >

    2. < element name =" adm " >

      1. < element name =" sets " >

        1. < element name =" hidden " >

          1. < element name =" none " >

            1. < field name =" value " > rc1 </ field >

            </ element >

          </ element >

        </ element >

      </ element >

    3. < element name =" bundles " >

      1. < element name =" bundle " >

        1. < field name =" name " > ORIGINAL </ field >

        2. < element name =" bitstreams " >

          1. < element name =" bitstream " >

            1. < field name =" name " > Nadal-et-al2005ChemBiochem_AM.pdf </ field >

            2. < field name =" originalName " > Nadal-et-al2005ChemBiochem_AM.pdf </ field >

            3. < field name =" description " />
            4. < field name =" format " > application/pdf </ field >

            5. < field name =" size " > 393430 </ field >

            6. < field name =" url " > https://dugi-doc.udg.edu/bitstream/10256/26011/4/Nadal-et-al2005ChemBiochem_AM.pdf </ field >

            7. < field name =" checksum " > a840d99d5f767546a57ab2e37c7e4374 </ field >

            8. < field name =" checksumAlgorithm " > MD5 </ field >

            9. < field name =" sid " > 4 </ field >

            </ element >

          </ element >

        </ element >

      2. < element name =" bundle " >

        1. < field name =" name " > SWORD </ field >

        2. < element name =" bitstreams " >

          1. < element name =" bitstream " >

            1. < field name =" name " > mets.xml </ field >

            2. < field name =" description " > SWORD deposit package </ field >

            3. < field name =" format " > application/zip </ field >

            4. < field name =" size " > 351817 </ field >

            5. < field name =" url " > https://dugi-doc.udg.edu/bitstream/10256/26011/3/mets.xml </ field >

            6. < field name =" checksum " > b021df7c1dc45fac977f8cd279bf4113 </ field >

            7. < field name =" checksumAlgorithm " > MD5 </ field >

            8. < field name =" sid " > 3 </ field >

            </ element >

          </ element >

        </ element >

      3. < element name =" bundle " >

        1. < field name =" name " > LICENSE </ field >

        2. < element name =" bitstreams " >

          1. < element name =" bitstream " >

            1. < field name =" name " > license.txt </ field >

            2. < field name =" originalName " > license.txt </ field >

            3. < field name =" format " > text/plain </ field >

            4. < field name =" size " > 1748 </ field >

            5. < field name =" url " > https://dugi-doc.udg.edu/bitstream/10256/26011/2/license.txt </ field >

            6. < field name =" checksum " > 8a4605be74aa9ea9d79846c1fba20a33 </ field >

            7. < field name =" checksumAlgorithm " > MD5 </ field >

            8. < field name =" sid " > 2 </ field >

            </ element >

          </ element >

        </ element >

      </ element >

    4. < element name =" others " >

      1. < field name =" handle " > 10256/26011 </ field >

      2. < field name =" identifier " > oai:dugi-doc.udg.edu:10256/26011 </ field >

      3. < field name =" lastModifyDate " > 2025-01-14 11:48:54.37 </ field >

      </ element >

    5. < element name =" repository " >

      1. < field name =" name " > DUGiDocs </ field >

      2. < field name =" mail " > oriol.olive@udg.edu </ field >

      </ element >

    6. < element name =" license " >

      1. < field name =" bin " > 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 </ field >

      </ element >

    </ metadata >

Biblioteca de Catalunya Carrer de l'Hospital, 56. 08001 Barcelona Adreça electrònica: catalonica@bnc.cat Tlf.: +34 932 702 300
  • Logotipo de Biblioteca de Catalunya
  • Logotipo de la Generalitat de Catalunya
  • Nota tècnica
  • Avís legal
  • Repositori OAI